Descriptive Page Image Leitz Calibration
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This page has the calibration data for the camera on our leitz microscope, and the data and images I used to generate it for those people that don't trust me.

The Calibration

1 pixel = 9.83 ± .03 µm
Objective Magnification

Or, for those of us too lazy to divide

Objective MagnificationPixel Size (µm)
5 1.966 ± .006
20 0.492 ± .002
50 0.1966 ± .0006
100 0.0983 ± .0003

Here's the stuff I used to generate the calibration.

Calibration Fit

After measuring the periodicity in pixels on a number of these images, and estimating errors on these measurements, I fit a simple scale factor using gnuplot. Fit and residuals are shown below. Clicking on an image will give a postscript file of that plot.

It may also be useful to look at the calibration images.

Calibration Plot
Residuals Plot